Faculty of Biology and Biotechnology

Publications

• Book

  Alshanskaia E., Martynova O.

  2023 Fifth International Conference Neurotechnologies and Neurointerfaces (CNN) 18-20 Sept. 2023

   Cognitive and emotional load in the course of increasing the complexity of tasks leads to the activation of various parts of the autonomic nervous system (ANS) and can be accompanied by an increase in the efficiency of problem solving. An increase in cognitive load under the condition of high motivation is a stress factor and is expressed in a different reaction of the sympathetic and parasympathetic links in response to the load. This study explored the ANS reactions by measuring the pupil size of 61 healthy sub-clinical volunteers in response to the gradually increasing complexity of tasks. The experiment included 3 blocks of a combined visual search and 1-back task with six levels of difficulty. K-mean clustering method was used for the analysis. An increase in the complexity of the task from the first to the sixth level led to different dynamics of the pupil size in the three clustered groups. In one group, an intense high switchability and an active reaction with a large amplitude of changes were visible, in the second group the dynamics of changes was minimal, and a third intermediate group was also identified. Our results highlight individual differences in the reaction of the sympathetic and parasympathetic branches of ANS under cognitive workload depending on biometric and psychometric parameters. 

  IEEE, 2023.

• Article

  Kryukova O. V., Islanov I. O., Zaklyazminskaya E. V. et al.

  Effects of Angiotensin-I-Converting Enzyme (ACE) Mutations Associated with Alzheimer’s Disease on Blood ACE Phenotype

  Backgrounds. Our recent analysis of 1200+ existing missense ACE mutations revealed that 400+ mutations are damaging and led us to hypothesize that carriers of heterozygous loss-of-function (LoF) ACE mutations (which result in low ACE levels) could be at risk for the development of late- onset Alzheimer’s disease (AD). Methods. Here, we quantified blood ACE levels in EDTA plasma from 41 subjects with 10 different heterozygous ACE mutations, as well as 33 controls, and estimated the effect of these mutations on ACE phenotype using a set of mAbs to ACE and two ACE substrates.
  Results. We found that relatively frequent (~1%) AD-associated ACE mutations in the N domain of ACE, Y215C, and G325R are truly damaging and likely transport-deficient, with the ACE levels in plasma at only ~50% of controls. Another AD-associated ACE mutation, R1250Q, in the cytoplasmic tail, did not cause a decrease in ACE and likely did not affect surface ACE expression. We have also developed a method to identify patients with anti-catalytic mutations in the N domain. These mutations may result in reduced degradation of amyloid beta peptide Aβ42, an important component for amyloid deposition. Consequently, these could pose a risk factor for the development of AD. Conclusions. Therefore, a systematic analysis of blood ACE levels in patients with all ACE mutations has the potential to identify individuals at an increased risk of late-onset AD. These individuals may benefit from future preventive or therapeutic interventions involving a combination of chemical and pharmacological chaperones, as well as proteasome inhibitors, aiming to enhance ACE protein traffic. This approach has been previously demonstrated in our cell model of the transport-deficient ACE mutation Q1069R.

  
  

  Biomedicines. 2024. Vol. 12. No. 10.

• Book chapter

  Панков Н. С., Pozanenko A. S., Belkin S. et al.

  AWARE: Alert Watcher and Astronomical Rapid Explorer

  We present the software aware for automatic scheduling the observations with the ground based network of optical telescopes GRB-IKI-FuN (extendable on other facilities). It was designed to provide the optimal coverage of large uncertainty contours of LIGO/Virgo/KAGRA (LVK) gravitational wave events, and gamma-ray bursts (GRBs), detected by space missions, which is crucial for fast look-up for their optical counterparts. In addition, aware provides capabilities for notifying users on new alert messages received from General Coordinate Network (GCN), and sharing final products generated by the planner via Telegram bot. The implementation of the software allows both scheduler, and Telegram bot to run concurrently in near real-time. In this paper, we discuss main follow-up tactics of LVK events, and GRBs. We overview the automatic planning software aware in terms of an architecture and algorithms. The example products made by aware since the beginning of the LVK O4 run on 25 May 2023 are given. The plans for future work are briefly discussed.

  In bk.: Data Analytics and Management in Data Intensive Domains: 25th International Conference, DAMDID/RCDL 2023, Moscow, Russia, October 24–27, 2023, Revised Selected Papers. Vol. 2086: Communications in Computer and Information Science. Springer, 2024. P. 225-245.

• Working paper

  Liutsko L., Malova Y., Vinokurova E. et al.

  Stress Resilience (Proprioceptive and Verbal Individual Differences) in Onco-Patients, Sportsmen and Controls

  The characteristics of response to stressful situations depend on a complex of factors such as personality traits, emotional regulation, health state, features of the nervous system, motor functions and proprioception. In cases of disruption of the brain's dopamine system due to disease or chemotherapy, hormone discharge due to stress, changes in emotions and proprioception may occur. The aim of our study was to find the relationship of stress resilence in verbal test and individual differences performed in proprioceptive test in onco-patients, sportsmen and group of control; as well as to observe the differences between these groups in the verbal stress resistance and individual differences proprioceptive tests. The results showed that onco-patients were less resistant as per verbal stress resistance outcomes for a major part of variables compared to other groups. The sportsmen were superior in verbal Risk and Control dimensions compared to other groups; but less in Positive Affectiveness. The proprioceptive test perfomance revealed the only statistically significant difference in performance in the dimension related to Mood, showing more Pessimism in the sportsmen group, but more Optimism in the oncopatients at temperamental (or intrisinc) level. The obtained results can be used by psychologists, therapists, coach and healthcare when needed to attend the specific needs of public, being pateints or sportsmen, etc.

  public health and health services. 20944. MDPI, 2023