HSE Researchers Identify Molecular Markers of Hypoxia in Preeclampsia
Researchers at the Molecular Physiology Laboratory of HSE University presented results of a multidisciplinary investigation into preeclampsia, correlating single-cell sequencing data from placental tissue in early-onset preeclampsia with experimental hypoxia induction in cell models. For the first time, gene expression profiles in diseased placental samples were directly compared to the molecular response of trophoblasts subjected to chemical hypoxia induction using a novel oxyquinoline derivative.
Innovative Methodology
The study integrated single-cell transcriptomics of placental cells with targeted hypoxia modeling via a new oxyquinoline-based HIF prolyl hydroxylase inhibitor. This approach activated oxygen-sensing pathways more specifically than traditional methods, revealing detailed molecular alterations across trophoblast subpopulations.
Key Findings
- Identification of common molecular markers of trophoblast hypoxia response
- Comparative analysis of multiple chemical hypoxia inducers
- Discovery of microRNA isoforms as potential early biomarkers
Translational Implications
These insights pave the way for novel diagnostic and therapeutic strategies for preeclampsia, a complication affecting 8% of pregnancies globally. The identified markers may support personalized early detection and monitoring of disease progression.
Bioinformatics Breakthrough
This work exemplifies the laboratory’s transition to advanced bioinformatics in reproductive pathology research. Combining single-cell sequencing with chemical modeling sets a new standard for comprehensive studies in maternal–fetal medicine.
The workshop covered topics related to preparing manuscripts for publication, including article structure, methodological aspects, and interpreting results for an international scientific audience.
Scientific Support
Funded by the Russian Science Foundation and the Basic Research Program at HSE University.